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KMID : 1098420190270060412
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Korean Journal of Medicinal Crop Science
2019 Volume.27 No. 6 p.412 ~ p.418
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Biological Activities and Separation of Active Substance of Extract and Fractions from Cotoneaster wilsonii Nakai Leaf
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Yoo Nam-Ho
Kim Hee-Kyu
Song Jae-Mo
Lee Chan-Ok
Park Ju-Hee f
Park Byung-Jun
Choi Yeong-Bin
Baek Yung-Sun
Hwang Yeon-Ji
Kim Myong-Jo
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Abstract
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Background: Previously, studies have observed that the leaf extract of Cotoneaster wilsonii Nakai has potent antioxidant and anti-inflammatory activites. Therefore, further research was conducted to separate the active antioxidant and anti-inflammatory compounds in the leaf of the C. wilsonii.
Methods and Results: The anti-oxidant effects were evaluated by analyzing the 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity, estimating the totla phenolic content (TPC) and total flavonoid content (TFC), and using reducing power assay method. The ethyl acetate (EtOAc) fraction demonstrated the highest DPPH radical scavenging activity (IC50; 9.69 ㎍/㎖), and the highest TPC and TFC (345.98 ㎎·GAE/g, and 74.23 ㎎·QE/g). Moreover, it indicated the highest effects on nitric oxide production. Therefore, the active compound was separated using the EtOAc fraction, determined on the basis of spectral data, and identified as (+)-catechin.
Conclusions: The first compound separated was made from the leaf of C. wilsonii was (+)-catechin, which produced potent anti-oxidant and anti-inflammatory effects.
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KEYWORD
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Cotoneaster wilsonii Nakai, Anti-oxidant, Anti-inflammatory, Catechin
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